lonA [2017-8-11 16:0:3]

class III heat-shock ATP-dependent protease

locus: BSU28200

pI: 5.77

mw: 86.42 kDa

protein length: 774 aa

gene length: 2322 bp

function: protein quality control, control of swarming motility

product: class III heat-shock ATP-dependent protease

essential: no

synonyms: lon

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.5|Additional chemotaxis signal transduction and regulatory proteins]

Gene

Coordinates: 2,880,466 → 2,882,790

Phenotypes of a mutant

• presence of predifferentiated swarmer cells in liquid medium [Pubmed|25538299]

The protein

Catalyzed reaction/ biological activity

• Hydrolysis of proteins in presence of ATP (according to Swiss-Prot)
• inhibits swarming motility by preventing accumulation of SwrA in liquid medium [Pubmed|25538299]

Protein family

• peptidase S16 family (according to Swiss-Prot)

Structure

• [PDB|5E7S] (active hexamer from ''Meiothermus taiwanensis'', 63% identity, 89% similarity) [Pubmed|27041593]
• [PDB|4YPN] (ADP-bound hexamer from ''Meiothermus taiwanensis'', 63% identity, 89% similarity) [Pubmed|27041592]
• [PDB|3M65] (N-terminal domain) [Pubmed|20600124]
• [PDB|3M6A] (C-terminal domain) [Pubmed|20600124]
• [PDB|1X37] (Ssd domain)

[SW|Localization]

• coincident with the nucleoid during normal growth and localized to the forespore during development [Pubmed|18689473]

Expression and Regulation

Operons

• genes: [gene|FF15BE26BCC78EC1301C58A51AF0A519D7BE9ADC|lonA]-[gene|DF1A043F3D9817D9479B8C707F4ACEEF2BF2862C|ysxC]
  description: [Pubmed|7961402]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7961402], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [Pubmed|9852015], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]

  regulation

  • induced by heat stress ([protein|search|CtsR]) [Pubmed|9852015]

References

Reviews

• 23479438,26639779

Original publications

• 7961403,7961402,19542270,19643080,9852015,18689473,20600124,25538299,27041592,27041593